LOCUS       40924_44928        5056 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC-TTrepT DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Vieira J, Messing J.
  TITLE     The pUC plasmids, an M13mp7-derived system for insertion mutagenesis
            and sequencing with synthetic universal primers
  JOURNAL   Gene 19 (3), 259-268 (1982)
  PUBMED    6295879
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Fujita A, Misumi Y, Koyama Y.
  TITLE     Two versatile shuttle vectors for Thermus thermophilus-Escherichia 
            coli containing multiple cloning sites, lacZalpha gene and kanamycin
            or hygromycin resistance marker
  JOURNAL   Plasmid 67 (3), 272-275 (2012)
  PUBMED    22252135
REFERENCE   3  (bases 1 to 5056)
  AUTHORS   Fujita A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2011) Contact:Atsushi Fujita National Institute of
            Advanced Science and Technology, Biomedical Research Institute; 
            1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan URL 
            :http://www.aist.go.jp/
REFERENCE   4  (bases 1 to 5056)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1982"; volume: "19"; issue: "3"; pages: "259-268"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "67"; issue: "3"; pages: "272-275"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (08-SEP-2011) Contact:Atsushi Fujita National Institute of Advanced 
            Science and Technology, Biomedical Research Institute; 1-1-1 
            Higashi, Tsukuba, Ibaraki 305-8566, Japan URL 
            :http://www.aist.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    233..283
                     /note="polylinker 
                     HindIII-NotI-SalI-NruI-AflII-EcoRV-Acc65I-EcoRI"
     primer_bind     complement(284..300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    690..3062
                     /label=derived from pYK225 (derivative of pTT8)
                     /note="derived from pYK225 (derivative of pTT8)"
     CDS             complement(1197..2360)
                     /codon_start=1
                     /gene="repA"
                     /product="putative RepA protein"
                     /label=repA
                     /protein_id="BAL70402.1"
                     /translation="MVLRAYAALRGLSPEALRAHLLAPPLRPERAREAFQRPYLAHFAQ
                     TLPRYPYATDDPKEGVRIYKRENALKRVHVQVGHYPHAVLRLVVDVDLPWPQVEERIHA
                     LPPSLVLVNPRSGHFHAWYELDPIPLTPPPGREGSLKGALALLAEVEALLEAYYGADPG
                     YNGLLSRNPFLHPPEWTWGGGKRWSLRDLHRELRGLLPSGTRRRVDPGLASYGRNNALF
                     DRLRAEAYAHVALFRGVPGGEEAFRAWVEQRAHALNQSLFRDHPKGPLDPREVHHTAKS
                     VAKWTYRNYRGARVYPVSSTGRPDRSRLSPQARALIPPLQGQELQEAVREGGRRRGSRR
                     RQEAEEKLTEALKRLQARGERVTARALAREAGVKPHTASKWLKRMRE"
     gene            complement(1197..2360)
                     /gene="repA"
                     /label=repA
     promoter        3150..3254
                     /label=AmpR promoter
     CDS             3255..4112
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4286..4874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
      241 cggccgcgtc gactcgcgac ttaagatatc cggtaccgaa ttcactggcc gtcgttttac
      301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      421 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      481 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      541 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      661 catcaccgaa acgcgcgaga cgaaagggcg gctatggagg ggttctccct gtctacgctt
      721 gagcgcccga gggccggtcc agcccccggg ccagagcggg ctccggaccc tgcccccaga
      781 aggaccctct cacccgtcct aaggccccta gaaggccggg gggcgggggg aggggtggga
      841 ttggtgcccc ccgcccaccc cccgccttaa aagccgtttt aggggcggtc ctcgaggggg
      901 aaaatcagtg tttttgccgt cctggacgat gaaaattgcg tgcgggcggg gtaaatatgt
      961 gagttatctc actttctctc ggatctcaag gtgtctaccc caagaaaaac aaaatttttg
     1021 cggcttccag gaaaaagaag ggggacttta gccgtcaact acgctaagga gactctctag
     1081 gttctccaat acagcccctt cggggctggc gggggttgca cccccgcacc cccgcctgta
     1141 taccctgata gccaccagct tggggcacat ttttggggtt tgtgccgtcc tggacgctac
     1201 tcccgcatcc tcttcaacca cttggaggcg gtatggggct tgacccccgc ctcccgggcc
     1261 agggccctgg ccgtgacccg ctccccccgg gcctggaggc gcttcagggc ctccgtgagc
     1321 ttctcctcgg cctcctgcct gcgccgggat ccgcgccgcc ttccgccctc ccgcaccgcc
     1381 tcctggagct cctggccctg gaggggcggg atcagggccc gggcctgggg agagaggcgg
     1441 ctccggtccg gcctccccgt ggaggagacc gggtagaccc tcgccccccg gtagttccgg
     1501 taggtccact tggccacgct cttcgccgtg tggtggacct cccgggggtc aaggggcccc
     1561 ttggggtggt cccggaagag ggactggttc agggcgtggg ccctctgctc cacccaggcc
     1621 cggaaggcct cctccccccc ggggacgccc cggaagaggg ccacgtgggc gtaggcctcc
     1681 gcccgcaggc ggtcaaacag ggcgttgttc cgcccgtagg aggccaggcc cgggtccacc
     1741 cgcctccggg tcccggaggg aaggagcccc cggagctccc ggtggaggtc ccgcaggctc
     1801 caccgcttcc ccccgcccca ggtccactcc ggggggtgga ggaaggggtt tcgggagagg
     1861 agaccgttgt agcccgggtc cgccccgtag taggcctcca gcagggcctc cacctccgcg
     1921 agaagggcca gggccccctt caggctcccc tcccgcccgg gcgggggcgt gagggggatg
     1981 gggtccagct cgtaccaggc gtggaagtgg cccgatctcg ggttgaccag gaccagggag
     2041 ggggggaggg cgtggatccg ctcctccacc tggggccagg ggaggtccac gtccaccacc
     2101 agccgcaaga cggcgtgggg gtagtggccc acctggacgt ggacccgctt cagggcgttc
     2161 tcccgcttgt agatgcgcac cccctccttg gggtcgtccg tggcgtaggg gtagcggggg
     2221 agagtctggg cgaagtgggc gaggtagggc cgctggaagg cctcccgggc ccgctccggg
     2281 cggaggggag gggccaggag gtgggcgcgg agggcctccg gggagaggcc gcgcagggcg
     2341 gcgtaggcgc gaagaaccac ctcccccagg tcggggttta tctggaagag tcctgcgatt
     2401 ttagcgaggg tgtccgggat ttgcgcttcc ggccgggtgt ggtgctccat ctcttgcctc
     2461 cttccccccg gccgggttag gatggaggcc tcctggggag gagaggcctc ggcggttaac
     2521 ccggccgagg tctgtttcat tcatgccccc cattctagcg acaagccccg ggaccaagcg
     2581 gtagtcccca ggtcaatgcc cccagaaccg ccaccagggc cgcctccgcg ggggctccag
     2641 ggccttcagg gcctcccgga ggcgggcgtt ctcctcccgc agggccctta gctccccctc
     2701 cacccgctcc agccgctcgg ccagggcccg gaggagggcc agggcctccc cctcggaggg
     2761 gagggcgagg gccgggaagt ctccctggac ccgggccagg gcctcctcca gggggaggcc
     2821 ttcccgcagg tgggcctccc gggccgcccg gaggcgggcc agggcctcct tgggccagag
     2881 ccgtcccccc cggggatccc ggggcagggg acctaccaac cgctcccaca gggcggcgta
     2941 gcgccggagg gtggccgggg agacccccag ggcccgggcg gccaaggccg ggggcaggag
     3001 ggtcgggtct tccccttccg ggtgctccac gaccccagtc tacccaggcg ctcagggtga
     3061 gcggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3121 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4981 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5041 gcgaggaagc ggaaga
//
